{"product_id":"pnc-27","title":"PNC-27","description":"\u003cdiv class=\"pb-product-desc\"\u003e\n\u003ch2\u003ePNC-27 — p53-Derived Membrane-Active Peptide for Cancer Biology Research\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003ePNC-27\u003c\/strong\u003e is a synthetic peptide derived from a segment of the p53 tumor suppressor protein (residues 12-26) fused to a transmembrane-penetrating sequence from the HDM-2 binding domain. Developed and characterized by Kanovsky et al. (2001) in \u003cem\u003eProceedings of the National Academy of Sciences\u003c\/em\u003e, PNC-27 has been shown to selectively bind to HDM-2 protein overexpressed on the surface of transformed cells, inducing rapid membranolysis through pore formation. This selectivity for malignant cells makes \u003cstrong\u003ePNC-27\u003c\/strong\u003e a unique tool for studying membrane dynamics and tumor-specific targeting strategies.\u003c\/p\u003e\n\n\u003ch3\u003eMechanism of Action\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003ePNC-27\u003c\/strong\u003e exploits the abnormal cell-surface expression of HDM-2 (human double minute 2) found on many transformed cell types. In non-malignant cells, HDM-2 is an intracellular protein that regulates p53 degradation via ubiquitination. However, in transformed cells, HDM-2 translocates to the outer membrane leaflet. PNC-27 binds this surface-exposed HDM-2 with high affinity, oligomerizes within the lipid bilayer, and forms transmembrane pores that cause rapid cell lysis. This mechanism operates independently of caspase-dependent apoptosis, inducing necrotic cell death within minutes of membrane engagement.\u003c\/p\u003e\n\n\u003ch3\u003eKey Research Areas\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSelective Membranolysis:\u003c\/strong\u003e In-vitro studies across multiple cancer cell lines (MDA-MB-231, MCF-7, PANC-1) have demonstrated that \u003cstrong\u003ePNC-27\u003c\/strong\u003e induces rapid membrane destruction in transformed cells while leaving non-malignant control cells (HEK-293, primary fibroblasts) viable at equivalent concentrations.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHDM-2 Surface Biology:\u003c\/strong\u003e PNC-27 has served as a probe for mapping HDM-2 externalization patterns, revealing that surface expression correlates with degree of malignant transformation and can be detected by flow cytometry using fluorescently labeled PNC-27 conjugates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCombination Studies:\u003c\/strong\u003e Research has investigated PNC-27 in combination with conventional chemotherapeutics, with published data suggesting synergistic cytotoxicity when paired with cisplatin or gemcitabine in pancreatic cancer models.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMembrane Pore Dynamics:\u003c\/strong\u003e Biophysical studies using artificial lipid bilayers have characterized PNC-27 pore formation kinetics, providing insights into peptide-membrane interaction mechanisms relevant to antimicrobial peptide design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDosage per vial:\u003c\/strong\u003e 5mg lyophilized powder\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e ≥98% (HPLC verified)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e -20°C, protected from light\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eWhy Researchers Choose Peptides BioLab\u003c\/h3\u003e\n\u003cp\u003eEvery batch of \u003cstrong\u003ePNC-27\u003c\/strong\u003e supplied by Peptides BioLab undergoes rigorous HPLC and mass spectrometry analysis, ensuring ≥98% purity verified by an independent Certificate of Analysis (CoA). Our product is supplied as a lyophilized powder for maximum stability, shipped worldwide in temperature-controlled packaging.\u003c\/p\u003e\n\n\u003ch3\u003eRelated Research Peptides\u003c\/h3\u003e\n\u003cp\u003eResearchers studying anti-tumor peptides and membrane biology may also find these compounds relevant: \u003ca href=\"\/products\/ll-37\"\u003eLL-37\u003c\/a\u003e, \u003ca href=\"\/products\/thymosin-alpha-1\"\u003eThymosin Alpha-1\u003c\/a\u003e, and \u003ca href=\"\/products\/bpc-157\"\u003eBPC-157\u003c\/a\u003e. For a complete overview, browse our \u003ca href=\"\/collections\/recovery\"\u003eRecovery Research\u003c\/a\u003e collection.\u003c\/p\u003e\n\n\u003cp\u003e\u003cem\u003eFor research purposes only — Not for human consumption. All products are intended solely for in-vitro research and laboratory use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Peptides BioLab","offers":[{"title":"Default Title","offer_id":61420737659210,"sku":"PBL-PNC27-5MG","price":44.99,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0998\/9429\/6906\/files\/peptides-biolab-pnc-27-peptide-5mg-research-grade.webp?v=1774393566","url":"https:\/\/peptides-biolab.com\/products\/pnc-27","provider":"Peptides BioLab","version":"1.0","type":"link"}