PNC-27
Lab-Tested ≥98% Purity · Certificate of Analysis Included · Global Shipping
PNC-27 — p53-Derived Membrane-Active Peptide for Cancer Biology Research
PNC-27 is a synthetic peptide derived from a segment of the p53 tumor suppressor protein (residues 12-26) fused to a transmembrane-penetrating sequence from the HDM-2 binding domain. Developed and characterized by Kanovsky et al. (2001) in Proceedings of the National Academy of Sciences, PNC-27 has been shown to selectively bind to HDM-2 protein overexpressed on the surface of transformed cells, inducing rapid membranolysis through pore formation. This selectivity for malignant cells makes PNC-27 a unique tool for studying membrane dynamics and tumor-specific targeting strategies.
Mechanism of Action
PNC-27 exploits the abnormal cell-surface expression of HDM-2 (human double minute 2) found on many transformed cell types. In non-malignant cells, HDM-2 is an intracellular protein that regulates p53 degradation via ubiquitination. However, in transformed cells, HDM-2 translocates to the outer membrane leaflet. PNC-27 binds this surface-exposed HDM-2 with high affinity, oligomerizes within the lipid bilayer, and forms transmembrane pores that cause rapid cell lysis. This mechanism operates independently of caspase-dependent apoptosis, inducing necrotic cell death within minutes of membrane engagement.
Key Research Areas
- Selective Membranolysis: In-vitro studies across multiple cancer cell lines (MDA-MB-231, MCF-7, PANC-1) have demonstrated that PNC-27 induces rapid membrane destruction in transformed cells while leaving non-malignant control cells (HEK-293, primary fibroblasts) viable at equivalent concentrations.
- HDM-2 Surface Biology: PNC-27 has served as a probe for mapping HDM-2 externalization patterns, revealing that surface expression correlates with degree of malignant transformation and can be detected by flow cytometry using fluorescently labeled PNC-27 conjugates.
- Combination Studies: Research has investigated PNC-27 in combination with conventional chemotherapeutics, with published data suggesting synergistic cytotoxicity when paired with cisplatin or gemcitabine in pancreatic cancer models.
- Membrane Pore Dynamics: Biophysical studies using artificial lipid bilayers have characterized PNC-27 pore formation kinetics, providing insights into peptide-membrane interaction mechanisms relevant to antimicrobial peptide design.
Technical Specifications
- Dosage per vial: 5mg lyophilized powder
- Purity: ≥98% (HPLC verified)
- Storage: -20°C, protected from light
Why Researchers Choose Peptides BioLab
Every batch of PNC-27 supplied by Peptides BioLab undergoes rigorous HPLC and mass spectrometry analysis, ensuring ≥98% purity verified by an independent Certificate of Analysis (CoA). Our product is supplied as a lyophilized powder for maximum stability, shipped worldwide in temperature-controlled packaging.
Related Research Peptides
Researchers studying anti-tumor peptides and membrane biology may also find these compounds relevant: LL-37, Thymosin Alpha-1, and BPC-157. For a complete overview, browse our Recovery Research collection.
For research purposes only — Not for human consumption. All products are intended solely for in-vitro research and laboratory use.
Every Peptides BioLab product includes a Certificate of Analysis (CoA) with HPLC and MS data confirming ≥98% purity. The CoA is available in the product tabs as the 2nd image, or contact our team to request a copy.
Shipping: We ship worldwide from our state-of-the-art facilities. Orders are processed within 1–2 business days. Express and standard shipping options available at checkout.
Storage: Store lyophilized peptides at -20°C for long-term storage. Once reconstituted, store at 2–8°C and use within 30 days. Keep away from direct sunlight.
